Determination of intracellular pH and compartmentation using diffusion-weighted NMR spectroscopy with pH-sensitive indicators
نویسندگان
چکیده
منابع مشابه
Electrodeless, accurate pH determination in highly basic media using a new set of (1)H NMR pH indicators.
A set of indicator molecules was selected and applied to elaborate an NMR-based pH determination method, free of glass electrode errors in highly basic media. Accurate measurement of pH values and protonation constants was achieved by a successive build-up of overlapping, increasingly high pH solutions, using a collection of 8 compounds of appropriately incremented basicities. In order to verif...
متن کاملMeasurement of intracellular pH of skeletal muscle with pH-sensitive glass microelectrodes.
We used three methods to examine the relationship among intracellular pH, transmembrane potential, and extracellular pH. Single-barreled electrodes permitted the determination of resting potential and intracellular pH with a minimum of cellular injury. Double-barreled electrodes, which incorporated a reference as well as a pH-sensitive electrode in a single tip, facilitated the direct measureme...
متن کامل19F NMR based pH probes: lanthanide(III) complexes with pH-sensitive chemical shifts.
Experimental measurements and theoretical analysis of magnetic properties, structural dynamics and acid-base equilibria for several lanthanide(III) complexes with tetraazacyclododecane derivatives as 19F NMR chemical shift pH probes are presented; pKa values vary between 6.9 and 7.7, with 18 to 40 ppm chemical shift differences between the acidic and basic forms for Ho(III) complexes possessing...
متن کاملIllumination of the Spatial Order of Intracellular pH by Genetically Encoded pH-Sensitive Sensors
Fluorescent proteins have been extensively used for engineering genetically encoded sensors that can monitor levels of ions, enzyme activities, redox potential, and metabolites. Certain fluorescent proteins possess specific pH-dependent spectroscopic features, and thus can be used as indicators of intracellular pH. Moreover, concatenated pH-sensitive proteins with target proteins pin the pH sen...
متن کاملMeasurement of intracellular pH in maize root tissue with a-methyl fluorinated alanines and in-vivo F NMR spectroscopy
Methods for the simultaneous measurement of vacuolar and cytoplasmic pH in plant tissues currently have significant limitations. This study demonstrates the usefulness of methyl difluoro alanine (F2ALA) and methyl trifluoro alanine (F3ALA) with in-vivo F NMR spectroscopy to measure vacuolar and cytoplasmic pH in maize root tissue. The pH dependence of the chemical shift of F2ALA and F3ALA is gr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Magnetic Resonance in Medicine
سال: 2004
ISSN: 0740-3194,1522-2594
DOI: 10.1002/mrm.20034